Mouse FCRL1/FcRH1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7286
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
CyTOF-ready, Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG1 Clone # 745916
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse FCRL1/FcRH1
Ala17-Ser219
Accession # Q8R4Y0
Ala17-Ser219
Accession # Q8R4Y0
Specificity
Detects mouse FCRL1/FcRH1 in direct ELISAs. In direct ELISAs, no cross-reactivity
with recombinant human FCRL1/FcRH1 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG1
Scientific Data Images for Mouse FCRL1/FcRH1 Antibody
Detection of FCRL1/FcRH1 in Mouse splenocytes by Flow Cytometry.
Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # FAB1217A) and either (A) Rat IgG1 Isotype Control (Catalog # MAB005) or (B) Rat Anti-Mouse FCRL1/FcRH1 Monoclonal Antibody (Catalog # MAB7286) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).Applications for Mouse FCRL1/FcRH1 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: Mouse splenocytes
Sample: Mouse splenocytes
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FCRL1/FcRH1
FCRL1 (Fc receptor-like protein 1; also CD307a, FcRH1 and IFGP1) is a 36 kDa (predicted) member of the Ig Superfamily. In mouse, it is found on mature follicular and marginal B cells, splenic T cells and NK cells. It would appear that mouse FCRL1 serves as an inhibiting coreceptor, in as much as it contains a series of cytoplasmic ITIM motifs. Mature mouse FCRL1 is a 327 amino acid (aa) type I transmembrane protein (aa 17-343). It contains a 203 aa extracellular region (aa 17‑219) that shows two C2-type Ig-like domains (aa 17-200), and a 103 aa cytoplasmic domain. There are multiple potential splice variants. One shows a deletion of aa 206-248 (potentially soluble), a second contains a six aa substitution for aa 319-343, a third possesses a three aa substitution for aa 209-343, and a fourth shows a 21 aa substitution for Ala11. Over aa 17-219, mouse FCRL1 shares 83% aa sequence identity with rat FCRL1. Over the same region, it shares 63% aa sequence identity with human FCRL1 when a third Ig-like domain that is present in human is excluded from the analysis.
Long Name
Fc Receptor-like 1
Alternate Names
BXMH1, CD307a, FcRH1, IFGP1, IRTA5
Gene Symbol
FCRL1
UniProt
Additional FCRL1/FcRH1 Products
Product Documents for Mouse FCRL1/FcRH1 Antibody
Product Specific Notices for Mouse FCRL1/FcRH1 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...
Loading...