Mouse FGFR4 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF2265
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry, Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse FGF R4
Leu19-Asp366
Accession # CAA42551
Leu19-Asp366
Accession # CAA42551
Specificity
Detects mouse FGF R4 in Western blots. In Western blots, approximately 35% cross‑reactivity with recombinant human (rh) FGF R4 is observed and less than 5% cross-reactivity with rhFGF R1 alpha (IIIb), rhFGF R1 alpha (IIIc), rhFGF R1 beta (IIIb), rhFGF R1 beta (IIIc), recombinant mouse (rm) FGF R2 alpha (IIIb), rmFGF R2 alpha (IIIc), rmFGF R2 beta (IIIb), rmFGF R2 beta (IIIc), rmFGF R5, and rmFGF R5 beta is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse FGFR4 Biotinylated Antibody
FGF R4 in Mouse Embryo.
FGF R4 was detected in immersion fixed frozen sections of mouse embryo (E11.5) using Goat Anti-Mouse FGF R4 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2265) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to the epidermis. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Mouse FGFR4 Biotinylated Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (E11.5)
Sample: Immersion fixed frozen sections of mouse embryo (E11.5)
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse FGF R4
Sample: Recombinant Mouse FGF R4
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FGFR4
References
- Umbhauer, M.J. et al. (2000) J. Cell Sci. 113:2865.
- Cai, J. (2002) Dev. Biol. 251:221.
Long Name
Fibroblast Growth Factor Receptor 4
Alternate Names
CD334, FGF R4
Gene Symbol
FGFR4
UniProt
Additional FGFR4 Products
Product Documents for Mouse FGFR4 Biotinylated Antibody
Product Specific Notices for Mouse FGFR4 Biotinylated Antibody
For research use only
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