Mouse FoxN1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB7295

R&D Systems, part of Bio-Techne
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MAB7295
MAB7295-SP
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Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 717008

View all FoxN1 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse FoxN1
Val2-Asp164
Accession # Q61575

Specificity

Detects mouse FoxN1 in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse FoxN1 Antibody

FoxN1 antibody in Mouse Liver by Immunohistochemistry (IHC-Fr).

FoxN1 in Mouse Liver.

FoxN1 was detected in perfusion fixed frozen sections of mouse liver using Rat Anti-Mouse FoxN1 Monoclonal Antibody (Catalog # MAB7295) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in hepatocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Mouse FoxN1 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse liver

Reviewed Applications

Read 1 review rated 5 using MAB7295 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FoxN1

Forkhead box N1 (FoxN1) is a 69 kDa transcriptional regulator that is primarily expressed in thymic epithelial cells and keratinocytes. FoxN1 function is required for proper T cell, hair follicle, and nail differentiation. Mutations in FoxN1 result in severe combined immunodeficiency (SCID/Nude). Mouse FoxN1 contains one DNA-binding forkhead domain (aa 271-367). Within amino acids 1 - 164, mouse FoxN1 shares 84% and 95% aa sequence identity with human and rat FoxN1, respectively.

Long Name

Forkhead Box N1

Alternate Names

RONU, WHN

Entrez Gene IDs

8456 (Human); 15218 (Mouse); 287469 (Rat)

Gene Symbol

FOXN1

UniProt

Q61575

Additional FoxN1 Products

Product Documents for Mouse FoxN1 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse FoxN1 Antibody

For research use only

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