Mouse Frizzled-3 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1001
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse, Rat
Applications
Validated:
Immunohistochemistry
Cited:
Immunohistochemistry, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-3
His23-Val157
Accession # Q61086
His23-Val157
Accession # Q61086
Specificity
Detects mouse Frizzled-3 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) Frizzled-1, rmFrizzled-4, rmFrizzled-6, rmFrizzled-7, and rmFrizzled-8 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse Frizzled-3 Antibody
Frizzled‑3 in Mouse Embryo.
Frizzled-3 was detected in immersion fixed frozen sections of mouse embryo (E11) using Mouse Frizzled-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1001) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Mouse Frizzled-3 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (E11)
Sample: Immersion fixed frozen sections of mouse embryo (E11)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Frizzled-3
Alternate Names
Frizzled3, FZD3
Gene Symbol
FZD3
UniProt
Additional Frizzled-3 Products
Product Documents for Mouse Frizzled-3 Antibody
Product Specific Notices for Mouse Frizzled-3 Antibody
For research use only
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