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Mouse Frizzled-9 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF2440

R&D Systems, part of Bio-Techne
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AF2440
AF2440-SP

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Bioassay, Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Frizzled-9
Leu24-Asp186
Accession # Q9R216

Specificity

Detects mouse Frizzled-9 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant mouse (rm) Frizzled-2, rmFrizzled-3, rmFrizzled-4, rmFrizzled-6, rmFrizzled-7, and rmFrizzled-8 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse Frizzled-9 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of embryonic mouse brain (E11)

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Frizzled-9

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Frizzled-9

Frizzled-9 belongs to the Frizzled family of seven-transmembrane receptors, which are signaling receptors for the family of secreted Wnt ligands. The amino-terminal extracellular cysteine-rich domains of human and mouse Frizzled-9 share 94% amino acid sequence homology.

Alternate Names

CD349, Frizzled9, FZD9

Entrez Gene IDs

8326 (Human); 14371 (Mouse)

Gene Symbol

FZD9

UniProt

Additional Frizzled-9 Products

Product Documents for Mouse Frizzled-9 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse Frizzled-9 Antibody

For research use only

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