Mouse Galectin-2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6667
Key Product Details
Species Reactivity
Validated:
Mouse
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 747307
Product Summary for Mouse Galectin-2 Antibody
Immunogen
E. coli-derived recombinant mouse Galectin-2
Ser2-Glu130
Accession # Q9CQW5
Ser2-Glu130
Accession # Q9CQW5
Specificity
Detects mouse Galectin-2 in direct ELISAs and Western blots. In Western blots, approximately 5%
cross-reactivity with recombinant human (rh) Galectin-2 is observed and no cross-reactivity
with rhGalectin-4, -8, -10, recombinant mouse Galectin-1, -3, -7, or -9 is
observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse Galectin-2 Antibody
Detection of Mouse Galectin‑2 by Western Blot.
Western blot shows lysates of mouse stomach tissue. PVDF membrane was probed with 0.2 µg/mL of Rat Anti-Mouse Galectin-2 Monoclonal Antibody (Catalog # MAB6667) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Galectin-2 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Galectin‑2 in Mouse Stomach.
Galectin-2 was detected in perfusion fixed frozen sections of mouse stomach using Rat Anti-Mouse Galectin-2 Monoclonal Antibody (Catalog # MAB6667) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counter-stained with hematoxylin (blue). Specific staining was localized to gastric pits. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Mouse Galectin-2 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse stomach
Sample: Perfusion fixed frozen sections of mouse stomach
Western Blot
0.2 µg/mL
Sample: Mouse stomach tissue
Sample: Mouse stomach tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Galectin-2
Galectin-2 (Gal-2; also LEG2, HL14 and L-14-II) is a 14-15 kDa member of the S-type (1-CRD type) lectin family of proteins. It is a potentially secreted, noncovalent homodimer that is expressed by stomach mucosal columnar epithelium, small intestine Goblet cells and keratinocytes. Galectin-2 is reported to bind to CD29, and initiate CD8+ T cell apoptosis. Structurally, it serves to bind nonsialylated poly-NAcetyllactosamine motifs. Mouse Galectin-2 is 130 amino acids (aa) in length. There is one galectin domain (aa 4-130) that contains one dimerization interface (aa 123-128). Full-length mouse Galectin-2 shares 86% and 66% aa identity with rat and human Galectin-2, respectively.
Alternate Names
GAL2, Galectin2, LGALS2
Gene Symbol
LGALS2
UniProt
Additional Galectin-2 Products
Product Documents for Mouse Galectin-2 Antibody
Product Specific Notices for Mouse Galectin-2 Antibody
For research use only
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