Mouse Galectin‑9 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF3535

R&D Systems, part of Bio-Techne
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AF3535
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

ELISA Detection, ELISA Development

Label

Biotin

Antibody Source

Polyclonal Goat IgG

View all Galectin-9 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Galectin-9 short isoform
Ala2-Thr353; aa148-178 deleted
Accession # O08573

Specificity

Detects mouse Galectin-9 in Western blots. In this format, less than 1% cross-reactivity with recombinant human (rh) Galectin‑2, rhGalectin‑8, recombinant mouse (rm) Galectin-1, rmGalectin-3, rmGalectin-4, and rmGalectin-7 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse Galectin‑9 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Galectin-9 Short Isoform

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Galectin-9

Galectin-9, also known as Ecalectin, belongs to the Galectin family (S-type lectins) of galactoside-binding proteins. It is a 36 - 39 kDa protein that lacks a secretion signal sequence, but can be secreted by a non-classical pathway. Galectin-9 is a tandem-repeat Galectin with two Galectin domains joined by a linker region. Two alternative spliced variants, a short and a long isoform, exist. Galectin-9 short isoform is expressed in a variety of tissues and cells, including fibroblasts, hepatocytes, endothelial cells and astrocytes. The long isoform, however, is expressed primarily in the intestine. Galectin-9 binds TIM-3 on Th1 cells, inducing apoptosis. Mouse Galectin-9 shares 69% and 85% amino acid sequence identity with human and rat Galectin-9, respectively.

Alternate Names

Ecalectin, GAL9, Galectin9, LGALS9

Entrez Gene IDs

3965 (Human); 16859 (Mouse)

Gene Symbol

LGALS9

UniProt

O08573

Additional Galectin-9 Products

Product Documents for Mouse Galectin‑9 Biotinylated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Galectin‑9 Biotinylated Antibody

For research use only

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