Mouse GDF-1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF858

R&D Systems, part of Bio-Techne
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AF858
AF858-SP
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BAF858
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Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all GDF-1 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse GDF-1
His238-Arg357
Accession # P20863

Specificity

Detects mouse GDF-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross‑reactivity with recombinant mouse (rm) GDF‑5 is observed and less than 1% cross‑reactivity with rmGDF-6 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse GDF-1 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse GDF-1
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GDF-1

Growth and Differentiation Factor 1 (GDF‑1) is a secreted protein in the TGF‑ beta superfamily (1). It is the functional equivalent of frog, zebrafish, and chick Vg1 (2, 3). Human GDF‑1 is synthesized as a preproprotein that produces an approximately 44 kDa proprotein after cleavage of a 29 amino acid (aa) signal peptide. Further proteolysis at a dibasic cleavage site produces a 224 aa propeptide and a secreted 119 aa, 12‑15 kDa mature peptide (4). Mature human GDF‑1 shares 81% aa sequence identity with mouse and rat GDF‑1. GDF‑1 is expressed in the developing and adult nervous system, particularly in neurons of the cerebral cortex and hippocampus (4, 5). It is required during embryogenesis for development of left/right axis asymmetry, anterior/posterior axis asymmetry, and the notochord (2, 3, 6, 7). It cooperates with Nodal and GDF‑3 in these activities and potentiates Nodal bioactivity through formation of a 27 kDa noncovalent GDF‑1‑Nodal heterodimer (3, 7, 8). The presence of Nodal is required for GDF‑1 induced effects (8). GDF‑1 signals through heteromeric receptor complexes containing Activin RIIA or Activin RIIB, ALK4 or ALK7, and an EGF‑CFC protein such as Cryptic or Cripto (7‑9). Loss of function mutations in GDF‑1 are associated with a variety of human cardiac development defects (10).

References

  1. Rider, C.C. and B. Mulloy (2010) Biochem. J. 429:1.
  2. Wall, N.A. et al. (2000) Dev. Biol. 227:495.
  3. Andersson, O. et al. (2007) Dev. Biol. 311:500.
  4. Lee, S.-J. (1991) Proc. Natl. Acad. Sci. 88:4250.
  5. Soderstrom, S. and T. Ebendal (1999) J. Neurosci. Res. 56:482.
  6. Rankin, C.T. et al. (2000) Nat. Genet. 24:262.
  7. Andersson, O. et al. (2006) Dev. Biol. 293:370.
  8. Tanaka, C. et al. (2007) Genes Dev. 21:3272.
  9. Cheng, S.K. et al. (2003) Genes Dev. 17:31.
  10. Karkera, J.D. et al. (2007) Am. J. Hum. Genet. 81:987.

Long Name

Growth Differentiation Factor 1

Alternate Names

GDF1

Entrez Gene IDs

2657 (Human); 14559 (Mouse)

Gene Symbol

GDF1

UniProt

P20863

Additional GDF-1 Products

Product Documents for Mouse GDF-1 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse GDF-1 Antibody

For research use only

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