Mouse GDF-6/BMP-13 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF855
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse GDF-6/BMP-13
Thr335-Arg454
Accession # P43028
Thr335-Arg454
Accession # P43028
Specificity
Detects mouse GDF-6/BMP-13 in direct ELISAs.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Mouse GDF-6/BMP-13 Antibody
GDF‑6/BMP‑13 in Mouse Embryo.
GDF-6/BMP-13 was detected in immersion fixed frozen sections of mouse embryo (E13.5) using Sheep Anti-Mouse GDF-6/ BMP-13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF855) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to dorsal neural tube. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.Applications for Mouse GDF-6/BMP-13 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (E13.5)
Sample: Immersion fixed frozen sections of mouse embryo (E13.5)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GDF-6/BMP-13
Growth Differentiation Factor 6 (GDF-6), also known as bone morphogenetic protein 13 (BMP-13) or cartilage-derived morphogenetic protein 2 (CDMP-2), is a member of the bone morphogenetic protein (BMP) family which belongs to the transforming growth factor beta (TGF-beta) superfamily. The mature GDF-6 with 120 amino acids is a homodimeric protein containing the characteristic seven conserved cysteine residues. GDF-5, GDF-6 and GDF-7, which share 80‑86% identity, define a new subgroup within the BMP family. Like other TGF-beta superfamily proteins, GDF-6 is highly conserved across species. At the amino acid sequence level, human and mouse GDF‑6 are 99% identical. It has been reported that GDF-6 has multiple functions including regulation of myogenesis, regulation of chondrogenesis, bone morphogenesis, and neuron differentiation and survival. GDF-6 response is mediated by the formation of hetero-oligomeric complexes of type I (BMPR-IB) and type II (BMPR-II or Activin R-II) sereine/threonine kinase receptors, and the activation of Smad proteins (Smad 1, 5, and 8).
References
- Storm, E.E. et al. (1994) Nature 368:639.
- Nishitoh, H. et al. (1996) J. Biol. Chem. 271:21345.
- Francis-West, P.H. et al. (1999) Development 126:1035.
- Massague, J. et al. (2000) Genes and Dev. 14:627.
- Inada, M. et al. (1996) BBRC, 222:317.
- Settle, S.H., Jr. et al. (2003) Dev. Biol. 254:116.
Long Name
Growth Differentiation Factor 6
Alternate Names
BMP-13, GDF6
Gene Symbol
GDF6
UniProt
Additional GDF-6/BMP-13 Products
Product Documents for Mouse GDF-6/BMP-13 Antibody
Product Specific Notices for Mouse GDF-6/BMP-13 Antibody
For research use only
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