Mouse Granzyme G Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1346
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 207639
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Granzyme G
Ile21-Leu248
Accession # P13366
Ile21-Leu248
Accession # P13366
Specificity
Detects mouse Granzyme G in direct ELISAs and Western blots. In Western blots, approximately 5% cross‑reactivity with recombinant mouse Granzyme D is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Applications for Mouse Granzyme G Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Mouse Granzyme G (Catalog # 1346-SE)
Sample: Recombinant Mouse Granzyme G (Catalog # 1346-SE)
Reviewed Applications
Read 1 review rated 5 using MAB1346 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Granzyme G
Granzyme G is a member of the Granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells (1). Together with Granzymes D, E and F, it is regulated through pregnancy and by IL‑2 and IL‑15 in granulated metrial gland cells (2). A human or rat counterpart of mouse Granzyme G has not been found. Like other Granzymes, mouse Granzyme G is not secreted as a zymogen but stored as a fully processed and activated enzyme in the cytoplasmic granules of CTL (3). It is synthesized as a 248 amino acid (aa) precursor with a 18 aa signal peptide and a 2 aa propeptide (3, 4).
References
- Smyth, M.J. et al. (1996) J. Leukoc. Biol. 60:555.
- Allen, M.P. and M. Nilsen-Hamilton (1998) J. Immunol. 161:2772.
- Jenne, D.E. et al. (1989) Biochemistry 28:7953.
- Kwon, B.S. et al. (1988) J. Exp. Med. 168:1839.
Alternate Names
GrzG, GZMG
Gene Symbol
GZMG
UniProt
Additional Granzyme G Products
Product Documents for Mouse Granzyme G Antibody
Product Specific Notices for Mouse Granzyme G Antibody
For research use only
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