Mouse Granzyme G Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1346
Key Product Details
Species Reactivity
Mouse
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Granzyme G
Ile21-Leu248
Accession # P13366
Ile21-Leu248
Accession # P13366
Specificity
Detects mouse Granzyme G in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross‑reactivity with recombinant mouse Granzyme D is observed and less than 5% cross-reactivity with recombinant human Granzyme H and recombinant mouse Granzyme B is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Mouse Granzyme G Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Granzyme G (Catalog # 1346-SE), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Granzyme G (Catalog # 1346-SE), see our available Western blot detection antibodies
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse Granzyme G (Catalog # 1346-SE)
Sample: Recombinant Mouse Granzyme G (Catalog # 1346-SE)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Granzyme G
Granzyme G is a member of the Granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells (1). Together with Granzymes D, E and F, it is regulated through pregnancy and by IL-2 and IL-15 in granulated metrial gland cells (2). Human or rat counterpart of mouse Granzyme G has not been found. Like other Granzymes, mouse Granzyme G is not secreted as a zymogen but stored as a fully processed and activated enzyme in the cytoplasmic granules of CTL (3). It is synthesized as a 248 amino acid precursor with a 18 amino acid signal peptide and a 2 amino acid propeptide (3, 4). The mature protein (residues 21‑248) is expressed and purified.
References
- Smyth, M.J. et al. (1996) J. Leukoc. Biol. 60:555.
- Allen, M.P. and M. Nilsen-Hamilton (1998) J. Immunol. 161:2772.
- Jenne, D.E. et al. (1989) Biochemistry 28:7953.
- Kwon, B.S. et al. (1988) J. Exp. Med. 168:1839.
Alternate Names
GrzG, GZMG
Gene Symbol
GZMG
UniProt
Additional Granzyme G Products
Product Documents for Mouse Granzyme G Antibody
Product Specific Notices for Mouse Granzyme G Antibody
For research use only
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