Mouse Heparan Sulfate 6-O-Sulfotransferase 3/HS6ST3 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5406
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Heparan Sulfate 6-O-Sulfotransferase/HS6ST3
Pro28-Trp470
Accession # NP_056635
Pro28-Trp470
Accession # NP_056635
Specificity
Detects mouse Heparan Sulfate 6-O-Sulfotransferase/HS6ST3 in direct ELISAs.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Mouse Heparan Sulfate 6-O-Sulfotransferase 3/HS6ST3 Antibody
Heparan Sulfate 6-O-Sulfotransferase 3/HS6ST3 in Mouse Brain .
Heparan Sulfate 6-O-Sulfotransferase 3/HS6ST3 was detected in perfusion fixed frozen sections of mouse brain (trigeminal ganglion) using Sheep Anti-Mouse Heparan Sulfate 6-O-Sulfotransferase 3/HS6ST3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5406) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Mouse Heparan Sulfate 6-O-Sulfotransferase 3/HS6ST3 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse brain (trigeminal ganglion)
Sample: Perfusion fixed frozen sections of mouse brain (trigeminal ganglion)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Heparan Sulfate 6-O-Sulfotransferase 3/HS6ST3
HS6ST3 (Heparin sulfate 6-O-sulfotransferase 3) is a 60-62 kDa member of the sulfotransferase 6 family of enzymes. It is one of three 6-O sulfotransferase isoforms, all of which essentially perform the same function, but all of which are the products of distinct genes. HS6ST3 is known to sulfate the #6 position of glucosamine sulfate that is linked to a 2-O-sulfated iduronic acid in heparin sulfate. The molecule shows a temporal expression pattern, being found in postnatal cerebellar Purkinje cells and cerebral cortical pyramidal cells in layers II/III and V. Mouse HS6ST3 is a 470 amino acid (aa) type II transmembrane glycoprotein. It contains a short four aa cytoplasmic N-terminus coupled to an extended 443 aa lumenal domain (aa 28-470). The lumenal domain contains a stem region (aa 28-83) that mediates oligomerization and Golgi localization, plus a sulfotransferase enzyme domain (aa 142-409). Cleavage within the stem region by beta-secretase generates a soluble form of HS6ST3. Over aa 28-470, mouse HS6ST3 shares 99% and 95% aa identity with rat and human HS6ST3, respectively.
Alternate Names
DKFZp761K2315, heparan sulfate 6-O-sulfotransferase 3
Gene Symbol
HS6ST3
UniProt
Additional Heparan Sulfate 6-O-Sulfotransferase 3/HS6ST3 Products
Product Documents for Mouse Heparan Sulfate 6-O-Sulfotransferase 3/HS6ST3 Antibody
Product Specific Notices for Mouse Heparan Sulfate 6-O-Sulfotransferase 3/HS6ST3 Antibody
For research use only
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