Mouse ICAM-2/CD102 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF774
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry, Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse ICAM‑2/CD102
Ser20-Gln222
Accession # P35330
Ser20-Gln222
Accession # P35330
Specificity
Detects mouse ICAM‑2/CD102 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human ICAM-2, recombinant mouse (rm) ICAM-1, rmMAdCAM-1, and rmICAM-5 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Mouse ICAM-2/CD102 Biotinylated Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse ovary
Sample: Perfusion fixed frozen sections of mouse ovary
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse ICAM-2/CD102 Fc Chimera (Catalog # 774-M2)
Sample: Recombinant Mouse ICAM-2/CD102 Fc Chimera (Catalog # 774-M2)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ICAM-2/CD102
Intercellular Adhesion Molecule-2 (ICAM-2, also known as CD102), a member of the immunoglobulin superfamily, binds the leukocyte integrins LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). ICAM-2 is constitutively expressed at high levels on vascular endothelial cells and lymphohematopoietic cells. ICAM-2 mediated adhesion has been shown to provide a co-stimulatory signal for T cell aggregation, NK cytotoxicity and NK cell migration. The mouse and human ICAM-2 proteins share 60% amino acid identity. Human LFA-1 binds equally well to human or mouse ICAM-2.
References
- Somersalo, K. et al. (1995) J. Biol. Chem. 270:8629.
- Hayflick, J. et al. (1998) Immunologic Res. 17:313.
- Xu, H. (1992) J. Immunol. 149:2650.
Long Name
Intercellular Adhesion Molecule 2
Alternate Names
CD102, ICAM2
Gene Symbol
ICAM2
UniProt
Additional ICAM-2/CD102 Products
Product Documents for Mouse ICAM-2/CD102 Biotinylated Antibody
Product Specific Notices for Mouse ICAM-2/CD102 Biotinylated Antibody
For research use only
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