Mouse IGFALS/ALS Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1436
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IGFALS/ALS
Thr28-Cys603
Accession # P70389
Thr28-Cys603
Accession # P70389
Specificity
Detects mouse IGFALS/ALS in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse IGFALS/ALS Antibody
Detection of Mouse IGFALS/ALS by Western Blot.
Western blot shows lysates of mouse spleen tissue, mouse liver tissue, and mouse bone marrow. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse IGFALS/ALS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1436) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for IGFALS/ALS at approximately 80-90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Mouse IGFALS/ALS Antibody
Application
Recommended Usage
Western Blot
0.25 µg/mL
Sample: Mouse spleen tissue, mouse liver tissue, and mouse bone marrow
Sample: Mouse spleen tissue, mouse liver tissue, and mouse bone marrow
Reviewed Applications
Read 1 review rated 5 using AF1436 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IGFALS/ALS
ALS (Acid labile subunit) is an 84-86 kDa glycoprotein member of the leucine rich repeat superfamily of molecules. It is secreted by hepatocytes in response to growth hormone stimulation, and forms a 140-150 kDa ternary complex with IGFBP3 (or IGFBP5) and IGF-I. This complex increases the half-life of IGF-I and retains it in the circulation for use in multiple tissues. Mature mouse ALS is 580 amino acids (aa) in length (aa 24-603). It contains twenty-one 20-23 aa LRRs that participate in protein-protein interactions. There are two potential isoform variants that show N-terminal extensions. One contains a 66 aa substitution for aa 1-5, while another possesses an 89 aa substitution again for aa 1-5. Over aa 28-603, mouse ALS shares 79% and 93% aa identity with human and rat ALS, respectively.
Long Name
IGF-binding Protein Acid Labile Subunit
Alternate Names
ACLSD, ALS
Gene Symbol
IGFALS
UniProt
Additional IGFALS/ALS Products
Product Documents for Mouse IGFALS/ALS Antibody
Product Specific Notices for Mouse IGFALS/ALS Antibody
For research use only
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