Mouse GR-1 positive blood cells were stained with Rat Anti-Mouse IGSF2/CD101 APC-conjugated Monoclonal Antibody (Catalog # FAB3368A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse IGSF2/CD101 APC-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Mouse GR-1 positive blood cells

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IGSF2/CD101

IGSF2, also known as CD101 and V7 is a 135-140 kDa member of the EWI family, Ig superfamily of molecules. It is a type I transmembrane glycoprotein that is expressed on dermal dendritic cells (DCs), granulocytes, monocytes, activated T cells, and Tregs. IGSF2 ligation is involved in T cell activation. On dermal DCs, CD101 ligation induces IL-10 expression, and on activated T cells, CD101 ligation blocks IL-2 expression, two effects that downregulate T cell activity. Notably, in mouse, CD101 expression on CD62L⁺⁺ Tregs identifies a population of cells that have potent suppressor activity. The extracellular domain of mouse shares 86% and 69% amino acid sequence identity with the ECD of rat and human CD101, respectively.

Long Name

Immunoglobulin Superfamily, Member 2

Alternate Names

CD101, Leukocyte Surface Protein, V7

Entrez Gene IDs

9398 (Human); 630146 (Mouse)

Gene Symbol

CD101

UniProt

A8E0Y8

Additional IGSF2/CD101 Products

Product Documents for Mouse IGSF2/CD101 APC-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Mouse IGSF2/CD101 APC-conjugated Antibody

For research use only

Citations
Reviews

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Flow Cytometry

Mouse IGSF2/CD101 APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB3368A

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all IGSF2/CD101 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse IGSF2/CD101 APC-conjugated Antibody

Detection of IGSF2/CD101 in Mouse GR-1 Positive Blood Cells by Flow Cytometry.