HEK293 human cell line transfected with (A) Mouse IGSF9 or (B) irrelevant protein, and eGFP was stained with Rat Anti-Mouse IGSF9 Monoclonal Antibody (Catalog # MAB9140) followed by APC-conjugated Goat anti-Rat IgG Secondary Antibody (Catalog # F0113). Quadrant markers were set based on Rat IgG2B Isotype Control Antibody (Catalog # MAB0061). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse IGSF9 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with Mouse IGSF9 and eGFP

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGSF9

IGSF9, also known as Dasm1, is an approximately 130 kDa transmembrane protein that plays a role in neuronal synapse maintenance and function (1). Mature mouse Dasm1 consists of a 714 amino acid (aa) extracellular domain (ECD) with 5 Ig-like domains and 2 fibronectin type-3 domains, a 21 aa transmembrane segment, and a 424 aa cytoplasmic domain (2). Within the ECD, mouse Dasm1 shares 90% and 96% aa sequence identity with human and rat Dasm1, respectively. Alternative splicing generates an additional isoform that is truncated following the fifth Ig-like domain. Dasm1 is expressed in the dorsal root and trigeminal ganglia, forebrain, cortex, dentate gyrus, pyramidal cells, Purkinje cells, and hippocampal CA1 interneurons (2-5). It localizes to dendrites, cell bodies, and post-synaptic densities (3, 6). Dasm1 functions as a homophilic adhesion protein that supports the maintenance of inhibitory synapses as well as inhibitory neurotransmission (5, 6).

References

Hansen, M. and P.S. Walmod (2013) Neurochem. Res. 38:1236.
Doudney, K. et al. (2002) Genomics 79:663.
Shi, S.-H. et al. (2004) Proc. Natl. Acad. Sci. USA 101:13341.
Mishra, A. et al. (2008) Mol. Cell. Biol. 28:2782.
Mishra, A. et al. (2014) J. Neurosci. 34:4187.
Shi, S.-H. et al. (2004) Proc. Natl. Acad. Sci. USA 101:13346.

Long Name

Immunoglobulin Superfamily, Member 9

Alternate Names

Dasm1, FP18798, IGSF9A, KIAA1355, Nrt1, Protein Turtle Homolog A, TUTLA

Entrez Gene IDs

57549 (Human); 93842 (Mouse); 304982 (Rat)

Gene Symbol

IGSF9

UniProt

Q05BQ1

Additional IGSF9 Products

Product Documents for Mouse IGSF9 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse IGSF9 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse IGSF9 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9140

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all IGSF9 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse IGSF9 Antibody

Detection of IGSF9 in HEK293 Human Cell Line Transfected with Mouse IGSF9 and eGFP by Flow Cytometry.