Mouse IL-17RD/SEF Antibody

R&D Systems, part of Bio-Techne | Catalog # AF2276

R&D Systems, part of Bio-Techne
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AF2276
AF2276-SP
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Biotin
BAF2276
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Western Blot

Cited:

Flow Cytometry, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all IL-17RD/SEF Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse IL‑17 RD/SEF
Gly28-Arg299
Accession # Q8JZL1

Specificity

Detects mouse IL-17 RD/SEF in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human IL-17 RD is observed and less than 1% cross-reactivity with recombinant mouse (rm) IL-17 RC and rmIL-17B R is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse IL-17RD/SEF Antibody

Detection of IL-17 RD/SEF antibody in HEK293 Human Cell Line transfected with Mouse IL-17 RD/SEF antibody by Flow Cytometry.

Detection of IL‑17 RD/SEF in HEK293 Human Cell Line transfected with Mouse IL-17 RD/SEF by Flow Cytometry.

HEK293 human cell line transfected with mouse IL-17 RD/SEF was stained with Goat Anti-MouseIL-17 RD/SEF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2276, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.
Detection of IL-17 RD/SEF antibody in bEnd.3 Mouse Cell Line antibody by Flow Cytometry.

Detection of IL‑17 RD/SEF in bEnd.3 Mouse Cell Line by Flow Cytometry.

bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Mouse IL-17 RD/SEF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2276, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Applications for Mouse IL-17RD/SEF Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: bEnd.3 mouse endothelioma cell line and HEK293 human cell line transfected with mouse IL-17 RD/SEF

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse lung and thymus

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse IL-17 RD/SEF (Catalog # 2276-ML)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17RD/SEF

Interleukin -17 receptor D (IL-17 RD), also known as SEF (similar expression to FGFs), is a type I transmembrane protein that is found in both the cytoplasm and plasma membrane (1‑5). The gene for this protein belongs to a synexpression group originally identified in zebrafish and SEF is expressed along with FGF-3, -8, sprouty-2 (SPRY2) and SPRY4 (6, 7). Due to the presence of an alternate start site, there is one transcript that potentially gives rise to two isoforms. The first is a full-length long form and the second an N-terminally truncated form (2, 5). The significance and expression pattern of the short form are uncertain. The membrane‑bound long form of mouse IL-17 RD is synthesized as a 738 amino acid (aa) precursor protein with a putative 27 aa signal peptide, a 272 aa extracellular domain, a 20 aa transmembrane segment and a 419 aa cytoplastic domain (5). The extracellular domain contains one Ig-like domain and a fibronectin type III motif. The cytoplasmic domain shares homology with the intracellular domains of IL-17 receptor family members and shows one TIR (Toll/IL-1 Receptor) domain and a putative TRAF6-binding motif (2). Natural IL-17 RD has been shown to form homomultimeric complexes (3). The full-length IL-17 RD isoform is expressed in most adult tissues and during embryonic development (3, 5). Functionally, IL-17 RD has been shown to be an inhibitor of FGF signaling. The molecule’s extracellular domain does not seem to be involved. There is an interaction between the intracellular domains of FGFR1/2 and IL-17 RD that blocks ERK dissociation from MEK, thereby interfering with downstream ERK activation of nuclear Elk-1 (8). IL-17 RD has also been reported to interact with TAK1 and induce JNK activation and apoptosis (9). Ligands that interact with the extracellular domain of IL-17 RD have not been identified.

References

  1. Furthauer, M. et al. (2002) Nat. Cell Biol. 4:170.
  2. Xiong, S. et al. (2003) J. Biol. Chem. 278:50273.
  3. Yang, R-B. et al. (2003) J. Biol. Chem. 278:33232.
  4. Preger, E. et al. (2003) Proc. Natl. Acad. Sci. USA 101:1229.
  5. Lin, W. et al. (2002) Mech. Dev. 113:163.
  6. Tsang, M. et al. (2002) Nat. Cell Biol. 4:165.
  7. Kovalenko, D. et al. (2003) J. Biol. Chem. 278:14087.
  8. Torii, S. et al. (2004) Dev. Cell 7:33.
  9. Yang, X. et al. (2004) J. Biol. Chem. 279:38099.

Long Name

Interleukin 17 Receptor D, Transcript Variant 3

Alternate Names

IL-17 RD, IL17RD, IL17RLM, SEF

Entrez Gene IDs

54756 (Human); 171463 (Mouse)

Gene Symbol

IL17RD

UniProt

Q8JZL1

Additional IL-17RD/SEF Products

Product Documents for Mouse IL-17RD/SEF Antibody

Product Datasheets

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Product Specific Notices for Mouse IL-17RD/SEF Antibody

For research use only

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