Mouse IL-17RE Alexa Fluor® 350-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB7997U
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Flow Cytometry
Label
Alexa Fluor 350 (Excitation = 346 nm, Emission = 442 nm)
Antibody Source
Monoclonal Rat IgG1 Clone # 944904
Product Specifications
Immunogen
Mouse myeloma cell line NSO-derived recombinant mouse IL-17 RE
Met1-His414
Accession # Q8BH06
Met1-His414
Accession # Q8BH06
Specificity
Detects mouse IL-17 RE in direct ELISAs.
Clonality
Monoclonal
Host
Rat
Isotype
IgG1
Applications for Mouse IL-17RE Alexa Fluor® 350-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
0.25-1 µg/106 cells
Sample: CHO Chinese hamster ovary cell line transfected with mouse IL-17 RE
Sample: CHO Chinese hamster ovary cell line transfected with mouse IL-17 RE
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-17RE
N‑terminal deletions or deletion/substitution of the transmembrane segment (3). IL‑17 RE is expressed on keratinocytes, mucosal epithelial cells, Th17 cells, and gamma/ delta T cells (4, 5). It associates with the widely expressed IL‑17 RA to form a heterodimeric receptor for IL-17C (4‑6). IL-17C binds to IL‑17 RE with high affinity and to
IL‑17 RA with low affinity (4, 5). IL‑17C expression is induced by inflammatory stimulation in colon and airway epithelial cells, keratinocytes, CD4+ T cells, macrophages, and dendritic cells (4, 6, 7‑9). It is up‑regulated in various chronic inflammatory diseases including psoriasis, cystic fibrosis, and chronic obstructive pulmonary disease (COPD) (7, 8, 10). IL‑17 RE is reciprocally down‑regulated in psoriatic lesions (10). The interaction of IL‑17C with IL‑17 RE promotes mucosal immunity through the induction of anti‑bacterial peptides and pro‑inflammatory cytokines and chemokines (4, 6, 8, 9). IL‑17C action supports the integrity of the colon epithelium following infection induced damage (4, 6, 11) but also contributes to psoriatic skin thickening and the progression of arthritis (4, 8, 9). IL‑17C is additionally up‑regulated in Th17 cell dependent autoimmunity (5). In this setting, it exacerbates disease severity by inducing Th17 cell production of IL‑17A, IL‑17F, IL‑22, CCR6, and CCL20 (5). The up‑regulation of IL‑17 RE in hepatocellular carcinoma is associated with poor prognosis (12).
References
- Pappu, R. et al. (2012) Trends Immunol. 33:343.
- Rubino, S.J. et al. (2012) Trends Immunol. 33:112.
- Li, T.S. et al. (2006) Cell. Signal. 18:1287.
- Ramirez-Carrozzi, V. et al. (2011) Nat. Immunol. 12:1159.
- Chang, S.H. et al. (2011) Immunity 35:611.
- Song, X. et al. (2011) Nat. Immunol. 12:1151.
- Pfeifer, P. et al. (2013) Am. J. Respir. Cell Mol. Biol. 48:415.
- Johnston, A. et al. (2013) J. Immunol. 190:2252.
- Yamaguchi, Y. et al. (2007) J. Immunol. 179:7128.
- Johansen, C. et al. (2009) Br. J. Dermatol. 160:319.
- Reynolds, J.M. et al. (2012) J. Immunol. 189:4226.
- Liao, R. et al. (2013) J. Exp. Clin. Cancer Res. 32:3.
Long Name
Interleukin 17 Receptor E
Alternate Names
IL-17 RE, IL17RE, Il25r
Gene Symbol
IL17RE
UniProt
Additional IL-17RE Products
Product Documents for Mouse IL-17RE Alexa Fluor® 350-conjugated Antibody
Product Specific Notices for Mouse IL-17RE Alexa Fluor® 350-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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