Detects mouse IL‑18 R alpha/IL‑1 R5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) IL-1 RI, rmIL-1 RII, recombinant human (rh) IL-1 R3, rhIL-1 R4, recombinant rat (rr) IL-1 R6, rmIL-1 R7, rhIL-1 R8, rhIL-1 R9, or rmSIGIRR is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG₁

Scientific Data Images for Mouse IL-18 R alpha/IL-1 R5 Antibody

Detection of IL-18 R alpha/IL-1 R5 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Rat Anti-Mouse IL-18 Ra/IL-1 R5 Monoclonal Antibody (Catalog # MAB1216, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0105B).

Applications for Mouse IL-18 R alpha/IL-1 R5 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: Mouse splenocytes

Western Blot

1 µg/mL
Sample: Recombinant Mouse IL-18 R alpha/IL-1 R5

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-18 R alpha/IL-1 R5

IL-18 R, also known as IL-18 R alpha, is a member of the IL-1 receptor family and has been designated IL-1 R5.

Long Name

Interleukin 18 Receptor alpha

Alternate Names

CD218a, IL-1 R5, IL-1 Rrp, IL-18 R1, IL-18Ra, IL18R alpha, IL18R1

Entrez Gene IDs

8809 (Human); 16182 (Mouse)

Gene Symbol

IL18R1

UniProt

Q61098

Additional IL-18 R alpha/IL-1 R5 Products

Product Documents for Mouse IL-18 R alpha/IL-1 R5 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse IL-18 R alpha/IL-1 R5 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse IL-18 R alpha/IL-1 R5 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB1216

Key Product Details

Species Reactivity

Validated:

Cited:

Applications