Mouse IL-1ra/IL-1F3 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF480
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
ELISA Detection (Matched Antibody Pair), Western Blot
Cited:
ELISA Development
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse IL‑1ra/IL-1F3
Specificity
Detects mouse IL‑1ra/IL-1F3 in ELISAs and Western blots. In sandwich immunoassays, approximately 45% cross-reactivity with recombinant rat IL‑1ra is observed and less than 1% cross-reactivity with recombinant human IL-1ra, recombinant porcine IL-1ra, and recombinant equine IL‑1ra is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Mouse IL-1ra/IL-1F3 Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse IL-1ra/IL-1F3 (Catalog # 480-RM)
Sample: Recombinant Mouse IL-1ra/IL-1F3 (Catalog # 480-RM)
Mouse IL-1ra/IL-1F3 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-1ra/IL-1F3
IL-1ra was originally isolated from the urine of patients with monocytic leukemia and has also been purified from adherent monocytes. The naturally-occurring, fully glycosylated form has an apparent molecular weight of about 25,000 Daltons. The protein shows 26% amino acid homology to IL-1 beta and 19% homology to IL-1 alpha. It will compete with either factor for receptor binding, but does not interact with either one. Human IL-1ra will bind to both types of IL-1 receptor (I and II) on human cells. In mouse, IL-1 RII does not bind IL-1ra. IL-1ra has been shown to block the inflammatory responses induced by IL-1 both in vitro and in vivo. Pre-clinical and clinical studies were done to test possible therapeutic applications for IL-1ra in the treatment of sepsis, rheumatoid arthritis and chronic myelogenous leukemia.
Long Name
Interleukin 1 Receptor Antagonist
Alternate Names
DIRA, ICIL-1ra, IL-1F3, IL-1ra3, IL-1RN, IL1ra, IL1RN, MVCD4
Gene Symbol
IL1RN
Additional IL-1ra/IL-1F3 Products
Product Documents for Mouse IL-1ra/IL-1F3 Biotinylated Antibody
Product Specific Notices for Mouse IL-1ra/IL-1F3 Biotinylated Antibody
For research use only
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