Mouse IL-21 Alexa Fluor® 488-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC594G
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Intracellular Staining by Flow Cytometry
Label
Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)
Antibody Source
Monoclonal Rat IgG2B Clone # 149204
Product Specifications
Immunogen
E. coli-derived recombinant mouse IL-21
Pro25-Ser146
Accession # Q9ES17
Pro25-Ser146
Accession # Q9ES17
Specificity
Detects mouse IL-21 in Western blots. Does not cross-react with recombinant human IL‑21, recombinant mouse (rm) IL‑15, or rmIL‑12.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Mouse IL-21 Alexa Fluor® 488-conjugated Antibody
Detection of IL-21 in Th17-stimulated Mouse Splenocytes by Flow Cytometry.
Th17-stimulated mouse splenocytes was stained with Rat Anti-Mouse IL-21 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC594G, filled histogram) or isotype control antibody (Catalog # IC013G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for Mouse IL-21 Alexa Fluor® 488-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
Sample: Th17-stimulated mouse splenocytes fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Sample: Th17-stimulated mouse splenocytes fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-21
Interleukin-21 (IL-21) and its receptor appear to play important roles in the regulation of the immune system. IL-21 is most related to IL-2, IL-4, and IL-15. IL-21 R, also called NILR (Novel Interleukin Receptor), is a type I cytokine receptor with 4 conserved cysteine residues and an extracellular WSXWS motif. It is most closely related to IL-2 R beta and IL-4 R alpha. Mouse IL-21 is a 146 amino acid (aa) residue protein with a 24 aa signal peptide. Mouse and human IL-21 share 57% aa identity. IL‑21 is expressed by activated T cells. Although not fully elucidated, the IL-2 R gamma ( gammac) chain appears to play a role in IL-21 R signaling. The IL-21/IL-21 R interaction appear to play important roles in B and T cell proliferation after antigen stimulation and NK cell maturation.
References
- Parrish-Novak, J. et al. (2000) Nature 408:57.
- Ozaki, K. et al. (2000) Proc. Natl. Acad. Sci. USA 97:11439.
- Dumoutier, L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:10144.
- Asao, H. et al. (2001) J. Immunol. 167:1.
Long Name
Interleukin 21
Alternate Names
CVID11, IL21, Za11
Gene Symbol
IL21
UniProt
Additional IL-21 Products
Product Specific Notices for Mouse IL-21 Alexa Fluor® 488-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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