Mouse IL-38/IL-1F10 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB77742
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Validated:
Mouse
Applications
ELISA
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rat IgG2A Clone # 798038R
Product Summary for Mouse IL-38/IL-1F10 Antibody
Immunogen
E. coli-derived recombinant mouse IL-38/IL-1F10
Met1-Arg152
Accession # Q8R459
Met1-Arg152
Accession # Q8R459
Specificity
Detects mouse IL-38/IL-1F10 in direct ELISAs.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse IL-38/IL-1F10 Antibody
Mouse IL‑38/IL‑1F10 ELISA Standard Curve.
Recombinant Mouse IL-38/IL-1F10 protein was serially diluted 2-fold and captured by Rat Anti-Mouse IL-38/IL-1F10 Monoclonal Antibody (Catalog # MAB77742) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse IL-38/IL-1F10 Monoclonal Antibody (Catalog # MAB7774R) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).Applications for Mouse IL-38/IL-1F10 Antibody
Application
Recommended Usage
ELISA
This antibody functions as an ELISA capture antibody when paired with Rat Anti-Mouse IL‑38/IL‑1F10 Monoclonal Antibody (Catalog # MAB7774R).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Mouse IL-38/IL1F10 DuoSet ELISA Kit (Catalog # DY2427-05) for convenient development of a sandwich ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C, as supplied.
- 1 month, 2 to 8 °C under sterile conditions after opening.
- 6 months, -20 to -70 °C under sterile conditions after opening.
Background: IL-38/IL-1F10
IL-1F10, also known as IL-1HY2, is a secreted protein that shares sequence and structural similarity with IL-1 receptor antagonist and other IL-1 family members. It is expressed in basal epithelia of the skin and in proliferating germinal center CD20+ B cells. IL-1F10 binds soluble IL-1RI in vitro and may participate in regulation of immune responses. Mouse IL-1F10 shares 82% and 93% aa identity with human and rat IL-1F10, respectively. IL-1F10 is predicted to be 17 kDa.
Long Name
Interleukin 1 Family Member 10
Alternate Names
FIL1- theta, FKSG75, IL-1HY2, IL1F10, IL38
Gene Symbol
IL1F10
UniProt
Additional IL-38/IL-1F10 Products
Product Documents for Mouse IL-38/IL-1F10 Antibody
Product Specific Notices for Mouse IL-38/IL-1F10 Antibody
For research use only
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