Mouse Integrin  alpha10 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9757

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Mouse

Applications

CyTOF-ready, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 885501

View all Integrin alpha 10 Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin alpha10 beta1
Phe23-Thr1119 (Integrin alpha10) and Gln21-Asp728 (Integrin beta1)
Accession # NP_001289400.1 (Integrin alpha10) and P09055 (Integrin beta1)

Specificity

Detects mouse Integrin alpha10 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Intagrin alpha2 beta1 was observered.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse Integrin  alpha10 Antibody

Detection of Integrin alpha 10 antibody in C2C12 Mouse Cell Line antibody by Flow Cytometry.

Detection of Integrin alpha 10 in C2C12 Mouse Cell Line by Flow Cytometry.

C2C12 mouse myoblast cell line was stained with Rat Anti-Mouse Integrin alpha 10 Monoclonal Antibody (Catalog # MAB9757, filled histogram) or Rat IgG2A isotype control antibody (Catalog # MAB006, open histogram) followed by Goat anti-Rat IgG APC-conjugated secondary antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse Integrin  alpha10 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: C2C12 mouse myoblast cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 10

Integrin alpha10 beta1 is one of twelve integrin family adhesion receptors that share the beta1 subunit (1‑3). The non‑covalent heterodimer of 160 kDa alpha11 and 130 kDa beta1/CD29 type I transmembrane glycoprotein subunits is expressed mainly on chondrocytes within cartilage, but also in fibrous connective tissues such as heart valves and ligaments (3, 4). The alpha10 extracellular domain (ECD) contains an I (inserted) domain which includes the ligand binding site (2, 3, 5). The beta1 ECD contains a vWFA domain, which participates in binding. Each subunit then has a transmembrane sequence and a short cytoplasmic tail. The dimer is folded when it is least active. Divalent cations and intracellular (inside‑out) signaling convert it to its most active, extended and open conformation (1, 2). The 1100 amino acid (aa) mouse  alpha10 extracellular domain (ECD) shares 96% aa sequence identity with  rat and 88‑89% with human, rabbit, porcine, canine and bovine alpha10, while the 708 aa mouse beta1 ECD shares 98% aa identity with rat and 93‑94% with human, bovine, porcine, ovine, canine and feline beta1. A potential mouse alpha10 splice variant diverges at aa 1039 and is terminated prematurely. If translated, this variant would result in a secreted protein (6). I domain‑containing beta1 integrins alpha1 beta1, alpha2 beta1, alpha10 beta1 and alpha11 beta1 all bind collagens; all but alpha11 beta1 also bind laminins (5, 7, 8). During cartilage differentiation, alpha10 beta1 is thought to be the main integrin binding type II and IX cartilage collagens (3‑5, 7‑10). However, deletion of mouse alpha10 causes a mild phenotype including slightly shortened bones and narrowed hypertrophic zones, indicating that another collagen‑binding integrin, likely alpha2 beta1, may compensate for alpha10 beta1 functions (11). Migration of melanoma cells has been noted to correlate with alpha10 beta1 expression (12).

References

  1. Takada, Y. et al. (2007) Genome Biol. 8:215.
  2. Luo, B-H. et al. (2007) Annu. Rev. Immunol. 25:619.
  3. Camper, L. et al. (1998) J. Biol. Chem. 273:20383.
  4. Camper, L. et al. (2001) Cell Tiss. Res. 306:107.
  5. Tulla, M. et al. (2001) J. Biol. Chem. 276:48206.
  6. Bengtsson, T. et al. (2001) Matrix Biol. 20:565.
  7. McCall-Culbreath, K.D. and M.M. Zutter (2008) Curr. Drug Targets 9:139.
  8. Popova, S.N. et al. (2007) Acta Physiol. 190:179.
  9. Varas, L. et al. (2007) Stem Cells Dev. 16:965.
  10. Gigout, A. et al. (2008) J. Biol. Chem. 283:31522.
  11. Bengtsson, T. et al. (2004) J. Cell Sci. 118:939.
  12. Wenke, A.K. et al. (2007) Cell Oncol. 29:373.

Alternate Names

ITGA10, PRO827

Entrez Gene IDs

8515 (Human); 213119 (Mouse); 310683 (Rat)

Gene Symbol

ITGA10

UniProt

NP_001289400.1 (Integrin alpha10) and P09055 (Integrin beta1)

Additional Integrin alpha 10 Products

Product Documents for Mouse Integrin  alpha10 Antibody

Product Datasheets

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Product Specific Notices for Mouse Integrin  alpha10 Antibody

For research use only

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