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Key Product Details

Species Reactivity

Mouse

Applications

CyTOF-ready, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 235033

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Integrin alpha2/CD49b
Tyr27-Thr1129
Accession # Q62469

Specificity

Detects mouse Integrin alpha2/CD49b in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Integrin alpha3, alpha4, alpha5, alphaE, or alphaV is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse Integrin  alpha2/CD49b Antibody

Detection of Integrin alpha 2/CD49b in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with (A) Rat Anti-Mouse Integrin a2/CD49b Monoclonal Antibody (Catalog # MAB1740) or (B) Rat IgG2B isotype control antibody (MAB006), followed by APC-conjugated Anti-Rat IgG Secondary Antibody (F0113) and Rat anti-Mouse NKp46 PE-conjugated Monoclonal Antibody (FAB22252P). Staining was performed using our Staining Membrane-associated Proteins protocol.

Applications for Mouse Integrin  alpha2/CD49b Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Mouse splenocytes

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 2/CD49b

The Integrin alpha2 subunit, also known as CD49b and VLA-2 alpha subunit, forms a heterodimer with the Integrin alpha1 (CD29) or alpha4 (CD104) subunits and binds Laminin.

Alternate Names

CD49b, ITGA2, VLA-2 alpha

Entrez Gene IDs

3673 (Human); 16398 (Mouse)

Gene Symbol

ITGA2

UniProt

Additional Integrin alpha 2/CD49b Products

Product Documents for Mouse Integrin  alpha2/CD49b Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse Integrin  alpha2/CD49b Antibody

For research use only

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