Detects mouse Integrin alpha7 in direct ELISAs. Reactivity with other Integrin alpha7 isoforms was not tested. In direct ELISAs, no cross-reactivity with recombinant mouse Integrin alpha2, alpha3, alpha4, alpha5, alpha9, alphaE, alphaX, recombinant human Integrin alphaL, or alpha11 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2B

Scientific Data Images for Mouse Integrin alpha7 Antibody

Detection of Integrin alpha7 in C2C12 Mouse Cell Line by Flow Cytometry.

C2C12 mouse myoblast cell line was stained with Rat Anti-Mouse Integrin a7 Monoclonal Antibody (Catalog # MAB3518, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse Integrin alpha7 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: C2C12 mouse myoblast cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 7

Integrin alpha7 is a transmembrane glycoprotein that forms heterodimers with Integrin beta1 to bind Laminin 1.

Alternate Names

ITGA7

Entrez Gene IDs

3679 (Human); 16404 (Mouse); 81008 (Rat); 102130741 (Cynomolgus Monkey)

Gene Symbol

ITGA7

UniProt

NP_032424

Additional Integrin alpha 7 Products

Product Documents for Mouse Integrin alpha7 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse Integrin alpha7 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse Integrin alpha7 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3518

Key Product Details

Species Reactivity

Validated:

Cited:

Applications