Mouse Integrin alphaE/CD103 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1990
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse, Transgenic Mouse
Applications
Validated:
CyTOF-ready, Flow Cytometry, Western Blot
Cited:
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Integrin alphaE/CD103
Phe20-Arg1113
Accession # Q60677
Phe20-Arg1113
Accession # Q60677
Specificity
Detects mouse Integrin alphaE/CD103 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse Integrin alphaE/CD103 Antibody
Detection of Integrin alphaE/CD103 in Mouse splenocytes by Flow Cytometry
Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Goat Anti-Mouse Integrin alphaE/CD103 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1990) or (B) isotype control antibody (Catalog # AB-108-C) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.Applications for Mouse Integrin alphaE/CD103 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: See below
Sample: See below
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse Integrin alphaE/CD103
Sample: Recombinant Mouse Integrin alphaE/CD103
Reviewed Applications
Read 1 review rated 5 using AF1990 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin alpha E/CD103
Alternate Names
CD103, HUMINAE, ITGAE
Gene Symbol
ITGAE
UniProt
Additional Integrin alpha E/CD103 Products
Product Documents for Mouse Integrin alphaE/CD103 Antibody
Product Specific Notices for Mouse Integrin alphaE/CD103 Antibody
For research use only
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