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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Flow Cytometry

Label

Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)

Antibody Source

Monoclonal Rat IgG2B Clone # M1/70

Product Specifications

Immunogen

Con A-activated C57BL/10 splenocytes

Specificity

Detects mouse Integrin alphaM/CD11b. Cross-reaction with human Integrin alphaM has been reported (1, 2).

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images

Detection of Integrin aM/CD11b antibody in Mouse Bone Marrow Cells antibody by Flow Cytometry.

Detection of Integrin alphaM/CD11b in Mouse Bone Marrow Cells by Flow Cytometry.

Mouse bone marrow cells were stained with Rat Anti-Mouse Gr-1/Ly-6G PE-conjugated Monoclonal Antibody (Catalog # FAB1037P) and either (A) Rat Anti-Mouse Integrin aM/CD11b Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB1124R) or (B) Rat IgG2BAlexa Fluor 647 Isotype Control (Catalog # IC013R). View our protocol for Staining Membrane-associated Proteins.

Applications

Application
Recommended Usage

Flow Cytometry

5 µL/106 cells
Sample: Mouse bone marrow cells

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD11b/Integrin alpha M

The Integrin family proteins are heterodimeric transmembrane receptors composed of an alpha and a beta subunit. The Integrin  alphaM subunit, also known as MAC-1 alpha subunit or CD11b, combines with the Integrin  beta2 subunit (CD18) to form the non-covalent heterodimer Integrin  alphaM/ beta2, also known as MAC-1 and Complement Receptor type 3 (CR3). Integrin  alphaM/ beta2 is expressed on granulocytes, macrophages, dendritic cells and natural killer cells. Upon activation, alphaM/ beta2 can bind several ligands (including ICAM-1, fibrinogen, and the C3 complement fragment, C3bi) to mediate phagocyte adhesion, migration and ingestion of complement-opsonized particles. The mouse extracellular domain (ECD) shares 87% and 74% amino acid sequence identity with rat and human ECD, respectively (3,4).

References

  1. Beller, D.J. et al. (1982) J. Exp. Med. 156:1000.
  2. Ault, K.A. and T.A. Springer (1981) J. Immunol. 126:359.
  3. Mitroulis, I. et al. (2015) Pharmacol. Ther. 147:123.
  4. Ross, G.D. et al. (2002) Immunol. Res. 25:219.

Alternate Names

CD11b, Integrin alpha M, ITGAM

Entrez Gene IDs

3684 (Human); 16409 (Mouse)

Gene Symbol

ITGAM

Additional CD11b/Integrin alpha M Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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