Mouse Intelectin-1/Omentin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB8074
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse, Bacteria - Helicobacter pylori, Transgenic Mouse
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 746420
Product Specifications
Immunogen
E. coli-derived recombinant mouse Intelectin‑1/Omentin
Ala20-Ser298
Accession # O88310
Ala20-Ser298
Accession # O88310
Specificity
Detects mouse Intelectin‑1/Omentin in ELISA. In direct ELISAs, no significant cross-reactivity with recombinant mouse Intelectin-2 and recombinant human Intelectin-1 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse Intelectin-1/Omentin Antibody
Detection of Mouse Intelectin‑1/Omentin by Western Blot.
Western blot shows lysates of mouse small intestine tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Intelectin-1/Omentin Monoclonal Antibody (Catalog # MAB8074) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Intelectin-1/Omentin at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Intelectin‑1/Omentin in Mouse Intestine.
Intelectin-1/Omentin was detected in perfusion fixed frozen sections of mouse intestine using Rat Anti-Mouse Intelectin-1/Omentin Monoclonal Antibody (Catalog # MAB8074) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to Paneth cells in intestinal glands. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.Applications for Mouse Intelectin-1/Omentin Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse intestine
Sample: Perfusion fixed frozen sections of mouse intestine
Western Blot
2 µg/mL
Sample: Mouse small intestine tissue
Sample: Mouse small intestine tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Intelectin-1/Omentin
Alternate Names
HL-1, Intelectin1, ITLN1, Itlna, LFR, Omentin, Omentin-1
Gene Symbol
ITLN1
UniProt
Additional Intelectin-1/Omentin Products
Product Documents for Mouse Intelectin-1/Omentin Antibody
Product Specific Notices for Mouse Intelectin-1/Omentin Antibody
For research use only
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