Mouse Intelectin-2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5358
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG1 Clone # 747421
Product Specifications
Immunogen
E. coli-derived recombinant mouse Intelectin-2
Ala20-Ser298
Accession # Q80ZA0
Ala20-Ser298
Accession # Q80ZA0
Specificity
Detects mouse Intelectin-2 in direct ELISAs and Western blots.
In direct ELISAs and Western blots, 100% cross-reactivity
with recombinant mouse (rm) Intelectin-1 is observed. In direct ELISAs, approximately 25%
cross-reactivity with recombinant human (rh) Intelectin-2 is observed, and
approximately 15% cross-reactivity with rhIntelectin-1 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG1
Scientific Data Images for Mouse Intelectin-2 Antibody
Detection of Mouse and Rat Intelectin‑2 by Western Blot.
Western blot shows lysates of mouse small intestine tissue and rat small intestine tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Intelectin-2 Monoclonal Antibody (Catalog # MAB5358) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Intelectin-2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Intelectin‑2 in Mouse Intestine.
Intelectin-2 was detected in perfusion fixed frozen sections of mouse intestine using Rat Anti-Mouse Intelectin-2 Monoclonal Antibody (Catalog # MAB5358) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counter-stained with hematoxylin (blue). Specific staining was localized to Paneth cells in intestinal glands. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Mouse Intelectin-2 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse intestine
Sample: Perfusion fixed frozen sections of mouse intestine
Western Blot
2 µg/mL
Sample: Mouse small intestine tissue and rat small intestine tissue
Sample: Mouse small intestine tissue and rat small intestine tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Intelectin-2
Alternate Names
HL-2, Intelectin-b, Intelectin2, Itln1b, ITLN2, Itlnb
Gene Symbol
ITLN2
UniProt
Additional Intelectin-2 Products
Product Documents for Mouse Intelectin-2 Antibody
Product Specific Notices for Mouse Intelectin-2 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...