Detects mouse Lgr5/GPR49 in ELISAs. Stains mouse Lgr5 transfected cells but not irrelevant transfectants in Flow Cytometry and Immunocytochemistry. This antibody, also known as "RD20", has been found to detect an epitope in C-terminal LRR cap of Lgr5 (Ref. 1).

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2B

Scientific Data Images for Mouse Lgr5/GPR49 PE-conjugated Antibody

Detection of Lgr5/GPR49 in HEK293 Human Cell Line Transfected with Mouse Lgr5/GPR49 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with either (A) mouse Lgr5/GPR49 or (B) irrelevant transfectants and eGFP was stained with Rat Anti-Mouse Lgr5/GPR49 PE-conjugated Monoclonal Antibody (Catalog # FAB8240P). Quadrant markers were set based on control antibody staining (Catalog # IC013P). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse Lgr5/GPR49 PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

0.25-1 µg/10⁶ cells
Sample: HEK293 human embryonic kidney cell line transfected with mouse Lgr5/GPR49 and eGFP

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Lgr5/GPR49

Leucine‑rich repeat G‑protein‑coupled receptor 5 (Lgr5), also called GPR49, is a 907 amino acid (aa), approximately 97 kDa (calculated), seven‑transmembrane glycoprotein receptor in the Lgr family of cell surface receptors. The subfamily of Lgrs comprising Lgr4, Lgr5, and Lgr6 are G‑protein‑independent mediators of the potentiating effect of R‑Spondins on Wnt signaling (2). Lgr5 binds and forms complexes with R‑Spondins, Frizzled Wnt receptors and LRP Wnt co‑receptors. The region of the mouse Lgr5 long extracellular domain used as an immunogen shares 90% and 95% amino acid sequence identity with human and rat Lgr5, respectively. Lgr5 is found on embryonic and adult epithelial stem cells (3). Lgr5⁺ stem cells can produce all epithelial cell types of the intestinal crypts (4). It is upregulated in stem cells that give rise to cancers such as intestinal, hepatocellular, pancreatic and ovarian carcinomas (5,6). This antibody has been referred to as "RD20" by Peng et al.(1).

References

Peng et al. (2013) Cell Rep. 3(6):1885.
de Lau et al. (2014) Genes Dev. 28(4):305.
Barker et al. (2013) Development 140(12):2484.
Clevers (2013) Nature 495(7439):53.
Wu et al. (2014) Nat Commun 5:3149.
Jang et al. (2013) PLoS One 8(12):e82390495.

Long Name

Leucine-rich Repeat Containing G Protein-coupled Receptor 5

Alternate Names

FEX, GPR49, GPR67, HG38

Entrez Gene IDs

8549 (Human); 14160 (Mouse); 299802 (Rat); 102120216 (Cynomolgus Monkey)

Gene Symbol

LGR5

UniProt

Q9Z1P4

Additional Lgr5/GPR49 Products

Product Documents for Mouse Lgr5/GPR49 PE-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Mouse Lgr5/GPR49 PE-conjugated Antibody

For research use only

Citations
Reviews

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Flow Cytometry

Mouse Lgr5/GPR49 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB8240P

Key Product Details

Species Reactivity

Validated:

Cited:

Applications