Mouse Lipin 3 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4064
Key Product Details
Species Reactivity
Mouse
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse Lipin 3
Thr428-Arg529
Accession # Q99PI4
Thr428-Arg529
Accession # Q99PI4
Specificity
Detects mouse Lipin 3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant human (rh) Lipin 3 is observed and less than 5% cross-reactivity with rhLipin 1 and rhLipin 2 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Mouse Lipin 3 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed CCD-18co human colon fibroblast cell line
Sample: Immersion fixed CCD-18co human colon fibroblast cell line
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse Lipin 3
Sample: Recombinant Mouse Lipin 3
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Lipin 3
Mouse Lipin 3 is a 94 kDa member of the small Lipin family of nuclear proteins. It is primarily expressed in the GI tract and may play a role in DAG production or membrane synthesis. Lipin 3 is 848 amino acids (aa) in length. It shows conserved N- and C-terminal domains that flank an NLS between aa 135‑144. Lipin 3 has modest phosphatidate phosphatase activity. Two potential isoforms exist; one shows a deletion of aa 507‑537, while the other shows a 10 aa insertion between Glu 506 and Ser 507. Over aa 428‑549, mouse Lipin 3 shares 84% and 96% aa sequence identity with human and rat Lipin 3, respectively.
Long Name
Phosphatidate Phosphatase LPIN3
Alternate Names
LIPN3L, LPIN3, SMP2
Gene Symbol
LPIN3
UniProt
Additional Lipin 3 Products
Product Documents for Mouse Lipin 3 Antibody
Product Specific Notices for Mouse Lipin 3 Antibody
For research use only
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