Mouse MDL-1/CLEC5A Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1639
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse MDL-1/CLEC5A
Tyr26-Lys190
Accession # AAI04365
Tyr26-Lys190
Accession # AAI04365
Specificity
Detects mouse MDL-1/CLEC5A in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human MDL-1/CLEC5A is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse MDL-1/CLEC5A Antibody
MDL‑1/CLEC5A in RAW 264.7 Mouse Cell Line.
MDL-1/CLEC5A was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Goat Anti-Mouse MDL-1/CLEC5A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1639) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI(blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Mouse MDL-1/CLEC5A Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed RAW 264.7 mouse monocyte/macrophage cell line
Sample: Immersion fixed RAW 264.7 mouse monocyte/macrophage cell line
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse MDL-1/CLEC5A
Sample: Recombinant Mouse MDL-1/CLEC5A
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MDL-1/CLEC5A
Long Name
Myeloid DAP12-associating Lectin
Alternate Names
CLEC5A, CLECSF5, MDL1
Gene Symbol
CLEC5A
UniProt
Additional MDL-1/CLEC5A Products
Product Documents for Mouse MDL-1/CLEC5A Antibody
Product Specific Notices for Mouse MDL-1/CLEC5A Antibody
For research use only
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