Mouse Meteorin Antibody

R&D Systems, part of Bio-Techne | Catalog # AF3475

R&D Systems, part of Bio-Techne
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AF3475
AF3475-SP
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Conjugate
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Biotin
BAF3475
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Rat

Applications

Validated:

Immunocytochemistry, Western Blot

Cited:

Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all Meteorin Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Meteorin isoform 1
Gly22-Asp291
Accession # Q8C1Q4

Specificity

Detects mouse Meteorin in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse Meteorin Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed rat cortical neural stem cells differentiated by growth factor withdrawal

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Meteorin (Catalog # 3475-MN)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Meteorin

Meteorin, named after meteor because this protein can transform glial cells into cells with an elongated tail, is a 30 kDa member of the Meteorin family (1). The secreted protein is also known as glial cell differentiation factor and hypoxia/reoxygenation regulatory factor. Mouse Meteorin is synthesized as a 291 amino acid (aa) precursor with a 21 aa signal sequence and a 270 aa mature chain.  Splicing variants produce two isoforms. Isoform 2 is missing residues 1‑164 found in isomer 1, and has a two aa substitution for aa 165‑166. Mouse Meteorin is 81% aa identical to human Meteorin (2). Meteorin is expressed in the central nervous system both during development and in adult mice (2). During development, Meteorin mRNA appears both in the central and peripheral nervous systems with the most prominent levels in neural progenitors, glial progenitors, and cells of the astrocyte lineage. In the adult mouse, Meteorin is detected only in the brain (2). Within the brain, the most prominent expression is found in the cerebellum where it is expressed by glial cells interspersed between Purkinje neurons (2). Meteorin is also detectable in several discrete neuronal populations, such as the superior colliculus, the ocular motor nucleus, the raphe and pontine nuclei, and various thalamic nuclei (2).Functionally, Meteorin plays important roles in both glial cell differentiation and axonal network formation during neurogenesis (1). In addition, research shows that when Meteorin is expressed and secreted by perivascular astrocytes, it upregulates thrombospondin-1/-2 to attenuate angiogenesis in the surrounding endothelial cells and to promote vascular maturation (3).

References

  1. Nishino, J. et al. (2004) EMBO J. 23:1998.
  2. Jorgensen, J.R. et al. (2009) J. Mol. Neurosci. March 4 Published online.
  3. Park, J.A. et al. (2008) Glia 56:247.

Alternate Names

METRN

Entrez Gene IDs

79006 (Human); 70083 (Mouse); 287151 (Rat)

Gene Symbol

METRN

UniProt

Q8C1Q4 (Mouse)

Additional Meteorin Products

Product Documents for Mouse Meteorin Antibody

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Product Specific Notices for Mouse Meteorin Antibody

For research use only

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