Mouse thymocytes were stained with Rat Anti-Mouse MS4A4B Monoclonal Antibody (Catalog # MAB4504, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0113).

Applications for Mouse MS4A4B Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Mouse thymocytes

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MS4A4B

MS4A4B, also called Chandra, is a member of the MS4A family of four-transmembrane proteins that includes CD20. It is expressed in immature CD4^-CD8^- T cells, and later in mature Th1 but not Th2 cells. MS4A4B is enriched in lipid rafts of activated T cells, and overexpression enhances Th1 cytokine expression, indicating a possible role in cell signaling. Mouse MS4A4B shares 69% overall amino acid (aa) identity with rat, and low (< 40%) aa identity with human MS4A4B.

Long Name

Membrane-spanning 4-domains, Subfamily A, Member 4B

Alternate Names

Chandra, Ly116

Entrez Gene IDs

60361 (Mouse)

Gene Symbol

MS4A4B

UniProt

NP_068364

Additional MS4A4B Products

Product Documents for Mouse MS4A4B Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse MS4A4B Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse MS4A4B Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4504

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all MS4A4B Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse MS4A4B Antibody

Detection of MS4A4B in Mouse Thymocytes by Flow Cytometry.