Mouse MUCDHL Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4159
Key Product Details
Species Reactivity
Mouse
Applications
CyTOF-ready, Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse MUCDHL
Gln29-Ala479
Accession # NP_082345
Gln29-Ala479
Accession # NP_082345
Specificity
Detects mouse MUCDHL in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human MUCDHL is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse MUCDHL Antibody
Detection of MUCDHL in Mouse Kidney Cells by Flow Cytometry.
Mouse kidney single-cell suspension was stained with Goat Anti-Mouse MUCDHL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4159, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).Applications for Mouse MUCDHL Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: Mouse kidney single-cell suspension
Sample: Mouse kidney single-cell suspension
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse MUCDHL
Sample: Recombinant Mouse MUCDHL
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MUCDHL
Mucin and cadherin-like protein (MUCDHL), also known as μ-protocadherin, is a 100 kDa type I transmembrane protein belonging to the protocadherin family of cell adhesion molecules. It is found apically on the brush border of renal proximal tubule epithelial cells. The extracellular domain of mature mouse MUCDHL contains four cadherin domains plus three repeat regions that constitute a mucin stalk (aa 541‑614). At least one splice variant exists in mouse that shows a deletion of aa’s 459‑620. Over aa’s 29‑479, mouse MUCDHL shares 63% and 79% aa identity with human and rat MUCDHL, respectively.
Long Name
Mucin And Cadherin-like
Alternate Names
CDHR5, MU-PCDH, MUPCDH
Gene Symbol
CDHR5
UniProt
Additional MUCDHL Products
Product Documents for Mouse MUCDHL Antibody
Product Specific Notices for Mouse MUCDHL Antibody
For research use only
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