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Mouse Netrin-G2a Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2744

R&D Systems, part of Bio-Techne
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MAB2744
MAB2744-SP

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 349719

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Netrin-G2a
Asp18-Arg504
Accession # NP_598007

Specificity

Detects mouse Netrin-G2a in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant mouse (rm) Netrin-1, rmNetrin-4, rmNetrin-G1a, recombinant chicken (rch) Netrin‑1, or rchNetrin-2 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Mouse Netrin-G2a Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse Netrin-G2a
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Netrin-G2a

Mouse Netrin-G2a is a member of a GPI-linked Netrin subfamily that is distantly related to classical Netrins. Netrin-G2a is approximately 485 aa in length. It contains an N-terminal laminin-related region plus a heparin-binding C-terminus that ends in a GPI-linkage. The laminin-related region shows a globular type VI domain followed by three variable-length EGF-like domains. Alternate splicing generates two larger isoforms. Amino acid insertions of 59 aa and 34 aa between EGF-like motifs 1 and 2 generate Netrin-G2b and Netrin-G2c, respectively. Mature mouse Netrin-G2a shares 96% and 99% aa identity with human Netrin-G2 and the equivalent region in rat Netrin-G2b, respectively.

Alternate Names

Lmnt2a, NetrinG2a, NTG2a

Entrez Gene IDs

84628 (Human); 171171 (Mouse)

Gene Symbol

NTNG2

UniProt

Additional Netrin-G2a Products

Product Documents for Mouse Netrin-G2a Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse Netrin-G2a Antibody

This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California.

U.S. Patent # 5,565,331, 6,096,866, 6,017,714, 6,309,638, 6,670,451, and other U.S. and international patents pending.

For research use only

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