Mouse Netrin-G2a Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB27441
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 349724
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Netrin-G2a
Asp18-Arg504
Accession # NP_598007
Asp18-Arg504
Accession # NP_598007
Specificity
Detects mouse Netrin-G2a in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) Netrin-G1a, rmNetrin-1, or rmNetrin-4 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Applications for Mouse Netrin-G2a Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse brain (hippocampus)
Sample: Perfusion fixed frozen sections of mouse brain (hippocampus)
Western Blot
1 µg/mL
Sample: Recombinant Mouse Netrin-G2a
Sample: Recombinant Mouse Netrin-G2a
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Netrin-G2a
Mouse Netrin-G2a is a member of a GPI-linked Netrin subfamily that is distantly related to classical Netrins. Netrin-G2a is approximately 485 aa in length. It contains an N-terminal laminin-related region plus a heparin-binding C-terminus that ends in a GPI-linkage. The laminin-related region shows a globular type VI domain followed by three variable-length EGF-like domains. Alternate splicing generates two larger isoforms. Amino acid insertions of 59 aa and 34 aa between EGF-like motifs 1 and 2 generate Netrin-G2b and Netrin-G2c, respectively. Mature mouse Netrin-G2a shares 96% and 99% aa identity with human Netrin-G2 and the equivalent region in rat Netrin-G2b, respectively.
Alternate Names
Lmnt2a, NetrinG2a, NTG2a
Gene Symbol
NTNG2
UniProt
Additional Netrin-G2a Products
Product Documents for Mouse Netrin-G2a Antibody
Product Specific Notices for Mouse Netrin-G2a Antibody
This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California.
U.S. Patent # 5,565,331, 6,096,866, 6,017,714, 6,309,638, 6,670,451, and other U.S. and international patents pending.
For research use only
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