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Mouse NPRA/NPR1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3974

R&D Systems, part of Bio-Techne
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MAB3974
MAB3974-SP

Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 488620

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse NPRA/NPR1
Ser29-Glu469 (predicted)
Accession # P18293

Specificity

Detects mouse NPRA/NPR1 in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Mouse NPRA/NPR1 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse kidney

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NPRA/NPR1

Mouse NPR1 (natriuretic peptide receptor-1; also ANP-R1 or guanlyl cyclase-A) is a 130 kDa member of the guanylyl cyclase family of proteins. It is a type I transmembrane glycoprotein that contains a 441 aa extracellular domain (ECD) (aa 29‑469) and a 567 aa cytoplasmic region. The cytoplasmic region shows a tyrosine kinase and guanylyl cyclase catalytic domain. NPR1 operates as an oligomer and binds both ANP (atrial natriuretic peptide) and BNP (B-type natriuretic peptide). ANP binding induces bronchodilation and a reduction in blood pressure. Over aa 29‑469, mouse NPR1 is 97% and 86% aa identical to rat and human NPR1, respectively.

Long Name

Natriuretic Peptide Receptor A

Alternate Names

ANPa, ANPRA, GC-A, GUCY2A, NPR1, NPRA, Pndr

Entrez Gene IDs

4881 (Human); 18160 (Mouse); 24603 (Rat)

Gene Symbol

NPR1

UniProt

Additional NPRA/NPR1 Products

Product Documents for Mouse NPRA/NPR1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse NPRA/NPR1 Antibody

For research use only

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