Mouse Plasminogen Kringle 5 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF742
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse Plasminogen Kringle 5
Cys481-Ala563
Accession # AAA50168
Cys481-Ala563
Accession # AAA50168
Specificity
Detects mouse Plasminogen Kringle 5 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human Angiostatin (Kringle 1‑3) is observed. This antibody is expected to show cross-reactivity with mouse plasminogen.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Mouse Plasminogen Kringle 5 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (E15)
Sample: Immersion fixed frozen sections of mouse embryo (E15)
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse Plasminogen Kringle 5
Sample: Recombinant Mouse Plasminogen Kringle 5
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Plasminogen Kringle 5
Plasminogen is a coenzyme that has five triple disulfide-linked kringle domains. It is converted to plasmin by plasminogen activators. Internal fragments of plasminogen, including the naturally occurring angiostatin isoforms and Kringle 5, can also be generated by proteolytic cleavage. These internal fragments have been shown to be potent angiogenesis inhibitors (1).
References
- Cao, Y. et al. (1997) J. Biol. Chem. 272:22924.
Alternate Names
HAE4
Gene Symbol
PLG
UniProt
Additional Plasminogen Kringle 5 Products
Product Documents for Mouse Plasminogen Kringle 5 Antibody
Product Specific Notices for Mouse Plasminogen Kringle 5 Antibody
For research use only
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