Mouse PlGF-2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB465
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse, Transgenic Mouse
Applications
Validated:
Blockade of Receptor-ligand Interaction, Western Blot
Cited:
Binding Assay, ELISA Development, ELISA Development (Detection), Immunohistochemistry, Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 62526
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse PlGF-2
Ala24-Pro158
Accession # P49764
Ala24-Pro158
Accession # P49764
Specificity
Detects mouse PlGF-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) PlGF or rhVEGF is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Applications for Mouse PlGF-2 Antibody
Application
Recommended Usage
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.015 - 0.06 μg/mL of this antibody will block 50% of the binding of 4 ng/mL of recombinant mouse PlGF-2 to immobilized recombinant Mouse VEGFR1/Flt-1 Fc Chimera coated at 2 μg/mL (100 µL/well). At 1 μg/mL, this antibody will block >90% of the binding.
Western Blot
1 µg/mL
Sample: Recombinant Mouse PlGF‑2 (Catalog # 465-PL) under non-reducing conditions only
Sample: Recombinant Mouse PlGF‑2 (Catalog # 465-PL) under non-reducing conditions only
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PlGF-2
Long Name
Placenta Growth Factor 2
Alternate Names
PlGF2
Gene Symbol
PGF
UniProt
Additional PlGF-2 Products
Product Documents for Mouse PlGF-2 Antibody
Product Specific Notices for Mouse PlGF-2 Antibody
For research use only
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