Mouse/Rat CD39L1/ENTPD2 Antibody

Detection of Mouse and Rat CD39L1/ENTPD2 by Western Blot.

Western blot shows lysates of mouse salivary gland tissue and rat placenta tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Mouse/Rat CD39L1/ENTPD2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5797) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD39L1/ENTPD2 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Detection of Mouse CD39L1/ENTPD2 by Immunocytochemistry/Immunofluorescence

NTPDase2 immunostaining co-localize with vimentin, desmin, and PDGF beta receptor in mice bladder.Cryosections of mouse bladders were labeled with antibodies to vimentin (A. green), desmin (D. green), PDGF beta receptor (G. green), and NTPDase2 (B. E. H. red) and Topro-3 to label nuclei (B. blue). Color merged panels are shown on the right (C. F. I). Merged signals of NTPDase2 and vimentin, desmin, and PDGF beta receptor are shown as yellow (C. F. I). White arrows indicate representative co-localization. White asterisks indicate non-co-localized signal of smooth muscle (I) and fibroblasts (F. I). White scale bars = 10 µm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23145014), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse CD39L1/ENTPD2 by Immunocytochemistry/Immunofluorescence

NTPDase2 co-localizes with CD34 but not tryptase in mouse bladder.Cryosections of mouse bladders were labeled with antibodies to CD34 (A, D. green), NTPDase2 (B. red), tryptase (E. red), c-kit (G. green), and Topro-3 to label nuclei (B. E. H. blue). Color merged panels are shown on the right (C, F, I). Merged signals are shown as yellow (C. F. I). White arrows indicate representative NTPDase2/CD34 co-localization (C) or CD34/tryptase co-localization (F); White asterisks indicate CD34 positive cells with no NTPDase2 staining (C). White scale bars = 10 µm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23145014), licensed under a CC-BY license. Not internally tested by R&D Systems.