Sheep IgG Horseradish Peroxidase-conjugated Antibody Best Seller
R&D Systems, part of Bio-Techne | Catalog # HAF016
Conjugate
Catalog #
Key Product Details
Species Reactivity
Sheep
Applications
Immunohistochemistry, Simple Western, Western Blot
Label
HRP
Antibody Source
Polyclonal Donkey IgG
Product Specifications
Immunogen
Sheep IgG
Specificity
Detects sheep IgG heavy and light chains in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with mouse IgG, rabbit IgG, human IgG, and chicken IgY is observed.
Clonality
Polyclonal
Host
Donkey
Isotype
IgG
Scientific Data Images for Sheep IgG Horseradish Peroxidase-conjugated Antibody
Detection of Sheep IgG Primary Antibody by Western Blot
Western blot shows detection of Sheep Anti-Human/Mouse/Rat PSMA1 Antigen Affinity-purified Polyclonal Antibody (AF7565) using a 1:1000 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Sheep IgG Primary Antibody by Simple WesternTM.
Simple Western lane view shows detection of Sheep Anti-Mouse Contactin-6 Polyclonal Antibody (AF5890) using a 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of AIF in Human Kidney
Detection of AIF in human kidney using 3 ug/mL of Sheep anti-human AIF primary antibody (AF5824) followed by 30 min incubation with Donkey anti Sheep HRP conjugated secondary antibodies (Catalog # HAF016) at 1:20 dilution + DAB chromogen (brown). The tissue was counterstained with Hematoxylin (blue). Control was done by omitting primary antibody.Applications for Sheep IgG Horseradish Peroxidase-conjugated Antibody
Application
Recommended Usage
Immunohistochemistry
Use at a 1:20 dilution
Simple Western
Use at a 1:50 dilution.
Western Blot
Use at a 1:1000 dilution.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 14 reviews rated 4.9 using HAF016 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Sodium Phosphate and Proclin 300.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Do not freeze.
- 6 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Long Name
Immunoglobulin G
Alternate Names
Immunoglobulin G, ImmunoglobulinG
Additional IgG Products
Product Documents for Sheep IgG Horseradish Peroxidase-conjugated Antibody
Product Specific Notices for Sheep IgG Horseradish Peroxidase-conjugated Antibody
For research use only
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