Mouse/Rat DARPP-32 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4230
Key Product Details
Species Reactivity
Validated:
Mouse, Rat
Cited:
Mouse, Transgenic Mouse
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunocytochemistry, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 375604
Product Specifications
Immunogen
E. coli-derived recombinant human DARPP-32
Ala51-Thr204
Accession # Q9UD71.2
Ala51-Thr204
Accession # Q9UD71.2
Specificity
Detects mouse and rat DARPP-32. Reactivity with human DARPP-32 has not been tested.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse/Rat DARPP-32 Antibody
Detection of Mouse/Rat DARPP-32 by Western Blot.
Western blot shows lysates of mouse brain corpus striatum tissue. PVDF membrane was probed with 1 µg/mL of Mouse/Rat DARPP-32 Monoclonal Antibody (Catalog # MAB4230) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for DARPP-32 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Mouse/Rat DARPP-32 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Perfusion fixed paraffin-embedded sections of rat brain (corpus striatum)
Sample: Perfusion fixed paraffin-embedded sections of rat brain (corpus striatum)
Western Blot
1 µg/mL
Sample: Mouse brain corpus striatum tissue
Sample: Mouse brain corpus striatum tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DARPP-32
Dopamine- and cAMP-Regulated Phosphoprotein, Mr 32 kDa (DARPP-32), also known as PPP1R1B, is a 23 kilodalton protein that anomalously migrates at about
32‑35 kDa on SDS-PAGE. When phosphorylated at T34 by protein kinase A (PKA), DARPP-32 is a potent inhibitor of protein phosphatase 1 (PP1). Dephosphorylation of DARPP-32 at T34 is achieved primarily by the calcium-dependent activation of the phosphatase calcineurin. DARPP-32 is expressed almost exclusively in neuronal tissues, with highest levels in dopamine-innervated neurons.
Long Name
Dopamine and cAMP-regulated Phosphoprotein
Alternate Names
DARPP32, PPP1R1B
Gene Symbol
PPP1R1B
UniProt
Additional DARPP-32 Products
Product Documents for Mouse/Rat DARPP-32 Antibody
Product Specific Notices for Mouse/Rat DARPP-32 Antibody
For research use only
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