Mouse/Rat MIF Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1978
Key Product Details
Species Reactivity
Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 811429
Product Specifications
Immunogen
E. coli-derived recombinant mouse MIF
Met1-Ala115
Accession # P34884
Met1-Ala115
Accession # P34884
Specificity
Detects mouse MIF in direct ELISAs. In direct ELISAs, no cross-reactivity
with recombinant human MIF is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse/Rat MIF Antibody
Detection of Mouse MIF by Western Blot.
Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line and J774A.1 mouse reticulum cell sarcoma macrophage cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse/Rat MIF Monoclonal Antibody (Catalog # MAB1978) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for MIF at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Rat MIF by Western Blot.
Western blot shows lysates of NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse/Rat MIF Monoclonal Antibody (Catalog # MAB1978) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for MIF at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Mouse/Rat MIF Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: RAW 264.7 mouse monocyte/macrophage cell line, J774A.1 mouse reticulum cell sarcoma macrophage cell line, and NR8383 rat alveolar macrophage cell line
Sample: RAW 264.7 mouse monocyte/macrophage cell line, J774A.1 mouse reticulum cell sarcoma macrophage cell line, and NR8383 rat alveolar macrophage cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MIF
MIF (Macrophage Inhibitory Factor; also GIF) is a 12-13 kDa member of the MIF family of proteins. Although secreted, it possesses no definitive signal sequence. MIF is widely expressed and serves to regulate immune reactions. On macrophages and B cells, MIF binds to a CD74:CD44 complex, initiating downstream signaling. On monocytes, this promotes TNF alpha production plus IFN-gamma -stimulated NO production. On B cells, this promotes B cell survival. MIF also binds to leukocyte CD74:CXCR2 and CD74:CXCR4 heterodimers, initiating T cell and monocyte migration. A tautomerase activity has also been shown for MIF and is suggested to involve the entire length of the molecule. Mouse MIF is 114 amino acids (aa) in length. MIF is phosphorylated at Ser91, and an additional, singular Cys residue is covalently linked to MIF at Cys60. MIF is considered to act as a homotrimer. Full-length mouse MIF shows 90% and 99% aa sequence identity with human and rat MIF, respectively.
Long Name
Macrophage Migration Inhibitory Factor
Alternate Names
EC 5.3.2.1, EC 5.3.3.12, GIFmacrophage migration inhibitory factor, GLIF, Glycosylation-inhibiting factor, L-dopachrome isomerase, L-dopachrome tautomerase, macrophage migration inhibitory factor (glycosylation-inhibiting factor), MMIF, Phenylpyruvate tautomerase
Gene Symbol
MIF
UniProt
Additional MIF Products
Product Documents for Mouse/Rat MIF Antibody
Product Specific Notices for Mouse/Rat MIF Antibody
For research use only
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