Mouse/Rat PRAT4B Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5015
Key Product Details
Species Reactivity
Validated:
Mouse, Rat
Cited:
Mouse
Applications
Validated:
CyTOF-ready, Flow Cytometry, Western Blot
Cited:
Immunocytochemistry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derive recombinant mouse PRAT4B
Thr30-Leu245
Accession # Q8BQ47
Thr30-Leu245
Accession # Q8BQ47
Specificity
Detects mouse and rat PRAT4B in Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human PRAT4B and recombinant mouse PRAT4A is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse/Rat PRAT4B Antibody
Detection of Mouse and Rat PRAT4B by Western Blot.
Western blot shows lysates of rat lung tissue and mouse spleen tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat PRAT4B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5015) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PRAT4B at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of PRAT4B in RAW264.7 Mouse Cell Line by Flow Cytometry
RAW 264.7 mouse monocyte/macrophage cell line was stained with Goat Anti-Mouse/Rat PRAT4B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5015, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).Applications for Mouse/Rat PRAT4B Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: RAW 264.7 mouse monocyte/macrophage cell line
Sample: RAW 264.7 mouse monocyte/macrophage cell line
Western Blot
1 µg/mL
Sample: Rat lung tissue and mouse spleen tissue
Sample: Rat lung tissue and mouse spleen tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PRAT4B
PRAT4B (protein associated with TLR4-B; also Cnpy4, protein canopy homolog 4) is a member of the canopy family of proteins. It is expressed by diverse cell types, including macrophages, fibroblasts and B cells. PRAT4B appears to be both secreted, and retained internally, where it may stabilize immature TLR protein expression in the ER, and impact its appearance on the cell surface. Mouse PRAT4B is 245 amino acids (aa) in length. It contains a 27 aa signal sequence plus one coiled-coil region (aa 209 - 237). There is one potential splice variant that shows a 13 aa substitution for the C-terminal nine amino acids. Over aa 30 - 245, mouse PRAT4B is 95% and 87% aa identical to rat and human PRAT4B, respectively.
Long Name
Protein Associated with Toll-like Receptor 4B
Alternate Names
CNPY4
Gene Symbol
CNPY4
UniProt
Additional PRAT4B Products
Product Documents for Mouse/Rat PRAT4B Antibody
Product Specific Notices for Mouse/Rat PRAT4B Antibody
For research use only
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