Mouse/Rat Tenascin R Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1624
Key Product Details
Species Reactivity
Validated:
Mouse, Rat
Cited:
Mouse, Rat
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunocytochemistry, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 619
Product Specifications
Immunogen
“rest L2” fraction from bovine brain
Specificity
Detects mouse and rat Tenascin R in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Mouse/Rat Tenascin R Antibody
Tenascin R in Rat Brain.
Tenascin R was detected in perfusion fixed frozen sections of rat brain using Mouse Anti-Mouse/Rat Tenascin R Monoclonal Antibody (Catalog # MAB1624) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of Mouse/Rat Tenascin R by Western Blot.
Western blot shows lysates of Mouse Brain and Rat Brain. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Mouse/Rat Tenascin R Monoclonal Antibody (Catalog # MAB1624) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Tenascin R at approximately 185-250 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.Applications for Mouse/Rat Tenascin R Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Perfusion fixed frozen sections of rat brain (hippocampus)
Sample: Perfusion fixed frozen sections of rat brain (hippocampus)
Western Blot
2 µg/mL
Sample: Mouse Brain and Rat Brain
Sample: Mouse Brain and Rat Brain
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 3 using MAB1624 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Tenascin R
Tenascin R is an extracellular matrix glycoprotein belonging to the Tenascin family of multidomain adhesion molecules. It is expressed exclusively in the central nervous system and affects neuronal cell migration and neurite extension. Mouse and rat Tenascin R share 97% amino acid sequence homology.
Alternate Names
Janusin, Restrictin, TNR
Gene Symbol
TNR
Additional Tenascin R Products
Product Documents for Mouse/Rat Tenascin R Antibody
Product Specific Notices for Mouse/Rat Tenascin R Antibody
For research use only
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