Mouse/Rat Thioredoxin-like 5/TRP14 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3769
Key Product Details
Species Reactivity
Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse Thioredoxin-like 5/TRP14
Met1-Asp123
Accession # Q9CQM5
Met1-Asp123
Accession # Q9CQM5
Specificity
Detects endogenous mouse and rat Thioredoxin-like 5/TRP14 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse/Rat Thioredoxin-like 5/TRP14 Antibody
Detection of Mouse/Rat Thioredoxin-like 5/TRP14 by Western Blot.
Western blot shows lysates of A20 mouse B cell lymphoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse/Rat Thioredoxin-like 5/TRP14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3769) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Thioredoxin-like 5/TRP14 at approximately 14 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.Applications for Mouse/Rat Thioredoxin-like 5/TRP14 Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: A20 mouse B cell lymphoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and Rat-2 rat embryonic fibroblast cell line
Sample: A20 mouse B cell lymphoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and Rat-2 rat embryonic fibroblast cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Thioredoxin-like 5/TRP14
Thioredoxin-like 5, also known as a 14 kDa thioredoxin-related protein (TRP14) is a 14 kDa cytosolic redox regulatory molecule that belongs to the thioredoxin family. It contains a thioredoxin-like redox-sensitive active site motif (Cys-Pro-Asp-Cys). In its reduced form, the active site cysteine residues reduce protein disulfide bonds. The resulting active site disulfide is subsequently reduced in a reaction catalyzed by the NADPH-dependent thioredoxin reductase 1. Thioredoxin-like 5 is ubiquitously expressed and acts on protein targets different from that of Thioredoxin-1. The amino acid sequence of mouse Thioredoxin-like 5 is 96% identical to that of rat Thioredoxin-like 5.
Alternate Names
Thioredoxin like 5, TRP14, TXNDC17, TXNL5
Gene Symbol
TXNDC17
UniProt
Additional Thioredoxin-like 5/TRP14 Products
Product Documents for Mouse/Rat Thioredoxin-like 5/TRP14 Antibody
Product Specific Notices for Mouse/Rat Thioredoxin-like 5/TRP14 Antibody
For research use only
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