Mouse/Rat TRH-degrading Ectoenzyme/TRHDE Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2985
Key Product Details
Species Reactivity
Validated:
Mouse, Rat
Cited:
Rat
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TRH-degrading Ectoenzyme/TRHDE
Arg64-His1025
Accession # Q8K093
Arg64-His1025
Accession # Q8K093
Specificity
Detects mouse and rat TRH-degrading Ectoenzyme/TRHDE in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse/Rat TRH-degrading Ectoenzyme/TRHDE Antibody
Detection of Mouse and Rat TRH-degrading Ectoenzyme/ TRHDE by Western Blot.
Western blot shows lysates of SH-SY5Y human neuroblastoma cell line and embryonic rat brain hippocampal glial . PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat TRH-degrading Ectoenzyme/TRHDE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2985) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TRH-degrading Ectoenzyme/TRHDE at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Mouse/Rat TRH-degrading Ectoenzyme/TRHDE Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: SH-SY5Y human neuroblastoma cell line and embryonic rat brain hippocampal glial
Sample: SH-SY5Y human neuroblastoma cell line and embryonic rat brain hippocampal glial
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TRH-degrading Ectoenzyme/TRHDE
TRHDE, also known as pyroglutamyl peptidase II and thyroliberinase, is a metalloprotease that specifically removes pyroglutamate from thyrotropin-releasing hormone, a tripeptide of L-pyroglutamyl-L-histidyl-L-prolineamide. TRH functions as a hypothalamic hypophysiotropic neuropeptide and neurotransmitter/neuromodulator within the central nervous system (1). Inhibitors of TRHDE have potential applications as research and therapeutic agents because TRHDE inactivates TRH (2). TRHDE is a type II transmembrane protein and a soluble form is also present in the serum (1). The recombinant mouse TRHDE corresponds to the ectodomain of the enzyme. Its amino acid sequence is 97% and 95% identical to that of rat and human.
References
- Schmitmeier, S. et al. (2002) Eur. J. Biochem. 269:1278.
- Kelly, J.A. et al. (2005) Biochem. J. 389:569.
Long Name
Thyrotropin Releasing Hormone-degrading Ectoenzyme
Alternate Names
PAP-II, PGPEP2, Thyroliberinase, TRH-DE, TRHDE, TRHdegrading Ectoenzyme
Gene Symbol
TRHDE
UniProt
Additional TRH-degrading Ectoenzyme/TRHDE Products
Product Documents for Mouse/Rat TRH-degrading Ectoenzyme/TRHDE Antibody
Product Specific Notices for Mouse/Rat TRH-degrading Ectoenzyme/TRHDE Antibody
For research use only
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