Mouse Renin 1 Propeptide Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4277

R&D Systems, part of Bio-Techne
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MAB4277
MAB4277-SP
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Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 434717

View all Renin Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Renin 1
Leu22-Arg402
Accession # P06281

Specificity

Detects mouse Renin 1 Propeptide in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with the mature form of mouse Renin 1, recombinant human Renin, recombinant mouse (rm) Cathepsin D, or rmCathepsin E is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Mouse Renin 1 Propeptide Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse Renin (Catalog # 4277-AS)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Renin

Mouse Renin is a secreted, 42‑47 kDa glycosylated member of the peptidase A1 family. It is an aspartyl protease that cleaves angiotensinogen to form angiotensin I. In mouse, there are two genes that code for Renin. One is in the submandibular gland and the other is in the kidney. The two mature Renin molecules are 95% amino acid (aa) identical. Renal Renin (Renin‑1) is synthesized as a 381 aa proform (aa 22‑402). In the kidney, pro‑Renin is proteolytically cleaved after Thr71 to generate a mature enzyme. Mouse pro‑Renin shares 70% and 85% aa sequence identity with human and rat pro‑Renin, respectively.

Alternate Names

Angiotensinogenase, REN

Entrez Gene IDs

5972 (Human); 19701 (Mouse)

Gene Symbol

REN

UniProt

P06281

Additional Renin Products

Product Documents for Mouse Renin 1 Propeptide Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Renin 1 Propeptide Antibody

For research use only

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