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Mouse Semaphorin 4A Alexa Fluor® 594-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB8166T

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Alexa Fluor 594 (Excitation = 590 nm, Emission = 617 nm)

Antibody Source

Monoclonal Rat IgG1 Clone # 757129

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Semaphorin 4A
Thr33-Trp638
Accession # Q62178

Specificity

Detects mouse Semaphorin 4A in ELISA. Stains HEK293 cells transfected with mouse Semaphorin 4A by Flow Cytometry, but does not stain untrasfected cells.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Applications for Mouse Semaphorin 4A Alexa Fluor® 594-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with mouse Semaphorin 4A and eGFP

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Semaphorin 4A

Semaphorin 4A (Sema4A, previously semB) is type I transmembrane protein that is a Class 4 transmembrane Semaphorin with activity in the immune and nervous systems. It is expressed by dendritic cells, monocytes, T cells, B cells, astrocytes and oligodendrocytes, plus visceral smooth muscle and bronchial epithelial cells. The 760 amino acid (aa) mouse Sema4A contains a 650 aa extracellular domain (ECD, aa 33-682) with Sema, PSI and C2-type immunoglobulin domains. It interacts with B and D1 Plexins and lymphocyte TIM-2, enhancing dendritic cell-mediated T cell priming and Th1 responses. Sema4A interaction with endothelial cell plexin-D1 downregulates the actions of VEGF. Mutations of Sema4A are associated with retinitis pigmentosa and cone rod dystrophy in humans. Mouse Sema4A ECD shares 87% and 94% aa sequence identity with human and rat Sema4A, respectively, and shares approximately 35% aa identity with other Sema4 family members. Using this antibody, Sema4A has been shown to interact with Neuropilin-1 in vitro and in vivo to potentiate Treg cell function and survival.

References

  1. Delgoffe, G.M. et al (2013) Nature 501:252.

Alternate Names

CORD10, SEMA4A, SEMAB, SEMB

Entrez Gene IDs

64218 (Human); 20351 (Mouse); 310630 (Rat)

Gene Symbol

SEMA4A

UniProt

Additional Semaphorin 4A Products

Product Documents for Mouse Semaphorin 4A Alexa Fluor® 594-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse Semaphorin 4A Alexa Fluor® 594-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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